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Promega plasmid pcat®3-control vector
Plasmid Pcat®3 Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+3-control/pcat+basic+plasmid/pm28233074-56-7-10
Average 90 stars, based on 1 article reviews
plasmid pcat®3-control vector - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Stable Transfection:

Article Title: Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression
Article Snippet: 21 pCAT 3-Control and pCAT 3-Basic were purchased from Promega (Madison, WI). pCH110 (a β-galactosidase-expressing vector) was from Pharmacia (Arlington Heights, IL).

Article Title: Enhanced expression of transgene in CHO cells using matrix attachment region.
Article Snippet: The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context.. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early promoter and flanked by two human b-globin MARs in cis.. We transfected this vector into the Chinese hamster ovary (CHO) cell line, and found that the level of CAT gene expression with MAR was effectively increased, about 5.493-fold higher than those without MARs.

Transfection:

Article Title: Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression
Article Snippet: 21 pCAT 3-Control and pCAT 3-Basic were purchased from Promega (Madison, WI). pCH110 (a β-galactosidase-expressing vector) was from Pharmacia (Arlington Heights, IL).

Article Title: Enhanced expression of transgene in CHO cells using matrix attachment region.
Article Snippet: The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context.. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early promoter and flanked by two human b-globin MARs in cis.. We transfected this vector into the Chinese hamster ovary (CHO) cell line, and found that the level of CAT gene expression with MAR was effectively increased, about 5.493-fold higher than those without MARs.

Plasmid Preparation:

Article Title: Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression
Article Snippet: 21 pCAT 3-Control and pCAT 3-Basic were purchased from Promega (Madison, WI). pCH110 (a β-galactosidase-expressing vector) was from Pharmacia (Arlington Heights, IL).

Article Title: Enhanced expression of transgene in CHO cells using matrix attachment region.
Article Snippet: The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context.. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early promoter and flanked by two human b-globin MARs in cis.. We transfected this vector into the Chinese hamster ovary (CHO) cell line, and found that the level of CAT gene expression with MAR was effectively increased, about 5.493-fold higher than those without MARs.

Generated:

Article Title: Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression
Article Snippet: 21 pCAT 3-Control and pCAT 3-Basic were purchased from Promega (Madison, WI). pCH110 (a β-galactosidase-expressing vector) was from Pharmacia (Arlington Heights, IL).

Article Title: Enhanced expression of transgene in CHO cells using matrix attachment region.
Article Snippet: The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context.. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early promoter and flanked by two human b-globin MARs in cis.. We transfected this vector into the Chinese hamster ovary (CHO) cell line, and found that the level of CAT gene expression with MAR was effectively increased, about 5.493-fold higher than those without MARs.

Amplification:

Article Title: Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression
Article Snippet: 21 pCAT 3-Control and pCAT 3-Basic were purchased from Promega (Madison, WI). pCH110 (a β-galactosidase-expressing vector) was from Pharmacia (Arlington Heights, IL).

Article Title: Enhanced expression of transgene in CHO cells using matrix attachment region.
Article Snippet: The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context.. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early promoter and flanked by two human b-globin MARs in cis.. We transfected this vector into the Chinese hamster ovary (CHO) cell line, and found that the level of CAT gene expression with MAR was effectively increased, about 5.493-fold higher than those without MARs.

Clone Assay:

Article Title: Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression
Article Snippet: 21 pCAT 3-Control and pCAT 3-Basic were purchased from Promega (Madison, WI). pCH110 (a β-galactosidase-expressing vector) was from Pharmacia (Arlington Heights, IL).

Article Title: Enhanced expression of transgene in CHO cells using matrix attachment region.
Article Snippet: The expression of transgenes in mammalian cells is often at a low level mainly due to position effects from the neighboring chromatin context.. To improve this, we have constructed a vector pCAM, which contains chloramphenicol acetyltransferase (CAT) reporter gene cassettes, driven by SV40 early promoter and flanked by two human b-globin MARs in cis.. We transfected this vector into the Chinese hamster ovary (CHO) cell line, and found that the level of CAT gene expression with MAR was effectively increased, about 5.493-fold higher than those without MARs.



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